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hindiii digested lambda phage dna  (New England Biolabs)


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    Structured Review

    New England Biolabs hindiii digested lambda phage dna
    Hindiii Digested Lambda Phage Dna, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 10794 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phage+%CE%BB+dna/HindIII/pmc13179615-117-16-20
    Average 99 stars, based on 10794 article reviews
    hindiii digested lambda phage dna - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Expressing:

    Article Title: Prokaryotic winged helix domains as dsDNA adenine methylation sensors
    Article Snippet: .. E. coli T7 expression strains C2566 (Dam + ) and its isogenic Dam-deficient strain ER2948 (constructed and provided by Dr. Lise Raleigh, New England Biolabs), expression vector pTXB1, pBR322, phage λ DNA (Dam + or Dam - ), 2-log (1 kb plus) DNA ladder, chitin beads, restriction enzymes, EcoGII methylase (frequent adenine methylase), Q5 DNA polymerase PCR master mix and cloning kit (Hi-Fi DNA assembly enzyme mix), NEBExpress Ni-NTA magnetic beads, and dZTP (2-aminoadenine triphosphate is abbreviated as base Z in the literature, in this paper we use dZ to differentiate from left-handed Z DNA) were provided by New England Biolabs (NEB). .. Ni-agarose beads were from Qiagen or NEB.

    Article Title: Cryo-EM structures of DNA-free and DNA-bound BsaXI: architecture of a Type IIB restriction-modification enzyme.
    Article Snippet: .. Competent cells of the cloning, expression and T7 expression strain C2566 (T7 Express), pUC19 plasmid, phage λ DNA, restriction enzymes, Phusion DNA polymerase, T4 polynucleotide kinase, Quick T4 DNA ligase, plasmid mini-prep kit, and DNA clean-up kit were provided by New England Biolabs (NEB). .. Deoxyoligonucleotides (oligos) and mutagenic primers for site-directed mutagenesis ( Supplementary Table S1 ) were purchased from IDT.

    Article Title: Characterization of winged helix domain fusion endonucleases as N6-methyladenine-dependent type IV restriction systems
    Article Snippet: .. E. coli T7 expression strains C2566 (Dam + ) and its isogenic Dam-deficient strain ER2948 [constructed and provided by Dr. Lise Raleigh, New England Biolabs (NEB)], expression vector pTXB1, pBR322, phage λ DNA (Dam + or Dam − ), 2-log (1 kb plus) DNA ladder, chitin beads, restriction enzymes, EcoGII methylase (frequent adenine methylase), Q5 DNA polymerase PCR master mix and cloning kit (Hi-Fi DNA assembly enzyme mix), NEBExpress Ni-NTA magnetic beads, and dZTP (2-aminoadenine triphosphate is abbreviated as base Z in the literature, here we use dZ to denote the 2’-deoxynucleoside) were provided by Michael Kuska (NEB Organic Synthesis Division). .. Fast-flow Ni-agarose beads were from Qiagen or NEB.

    Article Title: Cryo-EM structures of DNA-free and DNA-bound BsaXI: architecture of a Type IIB restriction–modification enzyme
    Article Snippet: .. Competent cells of the cloning, expression and T7 expression strain C2566 (T7 Express), pUC19 plasmid, phage λ DNA, restriction enzymes, Phusion DNA polymerase, T4 polynucleotide kinase, Quick T4 DNA ligase, plasmid mini-prep kit, and DNA clean-up kit were provided by New England Biolabs (NEB). .. Deoxyoligonucleotides (oligos) and mutagenic primers for site-directed mutagenesis ( ) were purchased from IDT.

    Construct:

    Article Title: Prokaryotic winged helix domains as dsDNA adenine methylation sensors
    Article Snippet: .. E. coli T7 expression strains C2566 (Dam + ) and its isogenic Dam-deficient strain ER2948 (constructed and provided by Dr. Lise Raleigh, New England Biolabs), expression vector pTXB1, pBR322, phage λ DNA (Dam + or Dam - ), 2-log (1 kb plus) DNA ladder, chitin beads, restriction enzymes, EcoGII methylase (frequent adenine methylase), Q5 DNA polymerase PCR master mix and cloning kit (Hi-Fi DNA assembly enzyme mix), NEBExpress Ni-NTA magnetic beads, and dZTP (2-aminoadenine triphosphate is abbreviated as base Z in the literature, in this paper we use dZ to differentiate from left-handed Z DNA) were provided by New England Biolabs (NEB). .. Ni-agarose beads were from Qiagen or NEB.

    Article Title: Characterization of winged helix domain fusion endonucleases as N6-methyladenine-dependent type IV restriction systems
    Article Snippet: .. E. coli T7 expression strains C2566 (Dam + ) and its isogenic Dam-deficient strain ER2948 [constructed and provided by Dr. Lise Raleigh, New England Biolabs (NEB)], expression vector pTXB1, pBR322, phage λ DNA (Dam + or Dam − ), 2-log (1 kb plus) DNA ladder, chitin beads, restriction enzymes, EcoGII methylase (frequent adenine methylase), Q5 DNA polymerase PCR master mix and cloning kit (Hi-Fi DNA assembly enzyme mix), NEBExpress Ni-NTA magnetic beads, and dZTP (2-aminoadenine triphosphate is abbreviated as base Z in the literature, here we use dZ to denote the 2’-deoxynucleoside) were provided by Michael Kuska (NEB Organic Synthesis Division). .. Fast-flow Ni-agarose beads were from Qiagen or NEB.

    Polymerase Chain Reaction:

    Article Title: Prokaryotic winged helix domains as dsDNA adenine methylation sensors
    Article Snippet: .. E. coli T7 expression strains C2566 (Dam + ) and its isogenic Dam-deficient strain ER2948 (constructed and provided by Dr. Lise Raleigh, New England Biolabs), expression vector pTXB1, pBR322, phage λ DNA (Dam + or Dam - ), 2-log (1 kb plus) DNA ladder, chitin beads, restriction enzymes, EcoGII methylase (frequent adenine methylase), Q5 DNA polymerase PCR master mix and cloning kit (Hi-Fi DNA assembly enzyme mix), NEBExpress Ni-NTA magnetic beads, and dZTP (2-aminoadenine triphosphate is abbreviated as base Z in the literature, in this paper we use dZ to differentiate from left-handed Z DNA) were provided by New England Biolabs (NEB). .. Ni-agarose beads were from Qiagen or NEB.

    Article Title: Characterization of winged helix domain fusion endonucleases as N6-methyladenine-dependent type IV restriction systems
    Article Snippet: .. E. coli T7 expression strains C2566 (Dam + ) and its isogenic Dam-deficient strain ER2948 [constructed and provided by Dr. Lise Raleigh, New England Biolabs (NEB)], expression vector pTXB1, pBR322, phage λ DNA (Dam + or Dam − ), 2-log (1 kb plus) DNA ladder, chitin beads, restriction enzymes, EcoGII methylase (frequent adenine methylase), Q5 DNA polymerase PCR master mix and cloning kit (Hi-Fi DNA assembly enzyme mix), NEBExpress Ni-NTA magnetic beads, and dZTP (2-aminoadenine triphosphate is abbreviated as base Z in the literature, here we use dZ to denote the 2’-deoxynucleoside) were provided by Michael Kuska (NEB Organic Synthesis Division). .. Fast-flow Ni-agarose beads were from Qiagen or NEB.

    Cloning:

    Article Title: Prokaryotic winged helix domains as dsDNA adenine methylation sensors
    Article Snippet: .. E. coli T7 expression strains C2566 (Dam + ) and its isogenic Dam-deficient strain ER2948 (constructed and provided by Dr. Lise Raleigh, New England Biolabs), expression vector pTXB1, pBR322, phage λ DNA (Dam + or Dam - ), 2-log (1 kb plus) DNA ladder, chitin beads, restriction enzymes, EcoGII methylase (frequent adenine methylase), Q5 DNA polymerase PCR master mix and cloning kit (Hi-Fi DNA assembly enzyme mix), NEBExpress Ni-NTA magnetic beads, and dZTP (2-aminoadenine triphosphate is abbreviated as base Z in the literature, in this paper we use dZ to differentiate from left-handed Z DNA) were provided by New England Biolabs (NEB). .. Ni-agarose beads were from Qiagen or NEB.

    Article Title: Cryo-EM structures of DNA-free and DNA-bound BsaXI: architecture of a Type IIB restriction-modification enzyme.
    Article Snippet: .. Competent cells of the cloning, expression and T7 expression strain C2566 (T7 Express), pUC19 plasmid, phage λ DNA, restriction enzymes, Phusion DNA polymerase, T4 polynucleotide kinase, Quick T4 DNA ligase, plasmid mini-prep kit, and DNA clean-up kit were provided by New England Biolabs (NEB). .. Deoxyoligonucleotides (oligos) and mutagenic primers for site-directed mutagenesis ( Supplementary Table S1 ) were purchased from IDT.

    Article Title: Characterization of winged helix domain fusion endonucleases as N6-methyladenine-dependent type IV restriction systems
    Article Snippet: .. E. coli T7 expression strains C2566 (Dam + ) and its isogenic Dam-deficient strain ER2948 [constructed and provided by Dr. Lise Raleigh, New England Biolabs (NEB)], expression vector pTXB1, pBR322, phage λ DNA (Dam + or Dam − ), 2-log (1 kb plus) DNA ladder, chitin beads, restriction enzymes, EcoGII methylase (frequent adenine methylase), Q5 DNA polymerase PCR master mix and cloning kit (Hi-Fi DNA assembly enzyme mix), NEBExpress Ni-NTA magnetic beads, and dZTP (2-aminoadenine triphosphate is abbreviated as base Z in the literature, here we use dZ to denote the 2’-deoxynucleoside) were provided by Michael Kuska (NEB Organic Synthesis Division). .. Fast-flow Ni-agarose beads were from Qiagen or NEB.

    Article Title: Cryo-EM structures of DNA-free and DNA-bound BsaXI: architecture of a Type IIB restriction–modification enzyme
    Article Snippet: .. Competent cells of the cloning, expression and T7 expression strain C2566 (T7 Express), pUC19 plasmid, phage λ DNA, restriction enzymes, Phusion DNA polymerase, T4 polynucleotide kinase, Quick T4 DNA ligase, plasmid mini-prep kit, and DNA clean-up kit were provided by New England Biolabs (NEB). .. Deoxyoligonucleotides (oligos) and mutagenic primers for site-directed mutagenesis ( ) were purchased from IDT.

    Magnetic Beads:

    Article Title: Prokaryotic winged helix domains as dsDNA adenine methylation sensors
    Article Snippet: .. E. coli T7 expression strains C2566 (Dam + ) and its isogenic Dam-deficient strain ER2948 (constructed and provided by Dr. Lise Raleigh, New England Biolabs), expression vector pTXB1, pBR322, phage λ DNA (Dam + or Dam - ), 2-log (1 kb plus) DNA ladder, chitin beads, restriction enzymes, EcoGII methylase (frequent adenine methylase), Q5 DNA polymerase PCR master mix and cloning kit (Hi-Fi DNA assembly enzyme mix), NEBExpress Ni-NTA magnetic beads, and dZTP (2-aminoadenine triphosphate is abbreviated as base Z in the literature, in this paper we use dZ to differentiate from left-handed Z DNA) were provided by New England Biolabs (NEB). .. Ni-agarose beads were from Qiagen or NEB.

    Article Title: Characterization of winged helix domain fusion endonucleases as N6-methyladenine-dependent type IV restriction systems
    Article Snippet: .. E. coli T7 expression strains C2566 (Dam + ) and its isogenic Dam-deficient strain ER2948 [constructed and provided by Dr. Lise Raleigh, New England Biolabs (NEB)], expression vector pTXB1, pBR322, phage λ DNA (Dam + or Dam − ), 2-log (1 kb plus) DNA ladder, chitin beads, restriction enzymes, EcoGII methylase (frequent adenine methylase), Q5 DNA polymerase PCR master mix and cloning kit (Hi-Fi DNA assembly enzyme mix), NEBExpress Ni-NTA magnetic beads, and dZTP (2-aminoadenine triphosphate is abbreviated as base Z in the literature, here we use dZ to denote the 2’-deoxynucleoside) were provided by Michael Kuska (NEB Organic Synthesis Division). .. Fast-flow Ni-agarose beads were from Qiagen or NEB.

    Plasmid Preparation:

    Article Title: Cryo-EM structures of DNA-free and DNA-bound BsaXI: architecture of a Type IIB restriction-modification enzyme.
    Article Snippet: .. Competent cells of the cloning, expression and T7 expression strain C2566 (T7 Express), pUC19 plasmid, phage λ DNA, restriction enzymes, Phusion DNA polymerase, T4 polynucleotide kinase, Quick T4 DNA ligase, plasmid mini-prep kit, and DNA clean-up kit were provided by New England Biolabs (NEB). .. Deoxyoligonucleotides (oligos) and mutagenic primers for site-directed mutagenesis ( Supplementary Table S1 ) were purchased from IDT.

    Article Title: Cryo-EM structures of DNA-free and DNA-bound BsaXI: architecture of a Type IIB restriction–modification enzyme
    Article Snippet: .. Competent cells of the cloning, expression and T7 expression strain C2566 (T7 Express), pUC19 plasmid, phage λ DNA, restriction enzymes, Phusion DNA polymerase, T4 polynucleotide kinase, Quick T4 DNA ligase, plasmid mini-prep kit, and DNA clean-up kit were provided by New England Biolabs (NEB). .. Deoxyoligonucleotides (oligos) and mutagenic primers for site-directed mutagenesis ( ) were purchased from IDT.



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    Image Search Results


    Recombinant M. hyorhinis MnuA2 recapitulates host tRNA cleavage pattern. ( A ) Purification of recombinant M. hyorhinis MnuA2. ( B ) M. hyorhinis MnuA2 shows strong RNase activity. SYBR Gold staining is shown. ( C, D ) The recombinant MnuA2 treatment (3 nM) shows the same cleavage pattern as that by live M. hyorhinis , and the MnuA2-induced tRNA cleavage is inhibited by EGTA treatment (5 mM) of the lysate. ( C ) SYBR Gold staining and ( D ) northern blotting for human tRNA Gly-GCC and tRNA iMet-CAT . Blue arrowheads indicate mature tRNAs, and red arrowheads indicate tDRs. ( E ) MnuA2 possesses both DNase and RNase activity. Linear dsDNA (1 µg λ DNA), circular dsDNA (1 µg pcDNA3.1(+)), or 5 µg total RNA was digested by 5 ng (roughly 1.05 nM) MnuA2. ( F ) M. hyorhinis lysate shows DNase activity at the same molecular weight range as the purified MnuA2 (indicated by a red arrowhead) in the presence of 3 mM CaCl 2 . Fifteen nanograms of MnuA2 corresponds to 0.32 pmol.

    Journal: Nucleic Acids Research

    Article Title: Cleavage of host tRNAs by mycoplasma membrane-associated nuclease

    doi: 10.1093/nar/gkag055

    Figure Lengend Snippet: Recombinant M. hyorhinis MnuA2 recapitulates host tRNA cleavage pattern. ( A ) Purification of recombinant M. hyorhinis MnuA2. ( B ) M. hyorhinis MnuA2 shows strong RNase activity. SYBR Gold staining is shown. ( C, D ) The recombinant MnuA2 treatment (3 nM) shows the same cleavage pattern as that by live M. hyorhinis , and the MnuA2-induced tRNA cleavage is inhibited by EGTA treatment (5 mM) of the lysate. ( C ) SYBR Gold staining and ( D ) northern blotting for human tRNA Gly-GCC and tRNA iMet-CAT . Blue arrowheads indicate mature tRNAs, and red arrowheads indicate tDRs. ( E ) MnuA2 possesses both DNase and RNase activity. Linear dsDNA (1 µg λ DNA), circular dsDNA (1 µg pcDNA3.1(+)), or 5 µg total RNA was digested by 5 ng (roughly 1.05 nM) MnuA2. ( F ) M. hyorhinis lysate shows DNase activity at the same molecular weight range as the purified MnuA2 (indicated by a red arrowhead) in the presence of 3 mM CaCl 2 . Fifteen nanograms of MnuA2 corresponds to 0.32 pmol.

    Article Snippet: Five nanograms of the MnuA2 were incubated at 37°C for the indicated time periods in 10 mM Tris-HCl (pH 8.0), 5 mM CaCl 2 , containing 1 μg λ phage DNA (TOYOBO), 1 μg pcDNA3.1(+) (Thermo Fisher Scientific), or 5 μg U2OS total RNA as a substrate.

    Techniques: Recombinant, Purification, Activity Assay, Staining, Northern Blot, Molecular Weight

    H262Q + H428Q double mutation completely abolishes the nuclease activity of MnuA2. ( A ) Conserved domains and putative catalytic sites of MnuA2, M. pulmonis MnuA ( WP_010925495.1 ) and mature ( N -terminal signal peptide-removed) human DNASE1 ( NP_005214.2 ). Amino acids comprising catalytic sites are indicated by red arrowheads, and two putative catalytic histidines are highlighted in red. ( B ) Purification of H262Q + H428Q mutant MnuA2 (mut-MnuA2). ( C, D ) Total RNA digestion by wild type (WT) or mutant (mut-) MnuA2 (3 nM) in the U2OS lysate. ( C ) SYBR Gold staining and ( D ) northern blotting for tRNA Gly-GCC . ( E ) in vitro DNA digestion by MnuA2. λ DNA and pcDNA3.1(+) were digested by 5 ng (roughly 1.05 nM) MnuA2.

    Journal: Nucleic Acids Research

    Article Title: Cleavage of host tRNAs by mycoplasma membrane-associated nuclease

    doi: 10.1093/nar/gkag055

    Figure Lengend Snippet: H262Q + H428Q double mutation completely abolishes the nuclease activity of MnuA2. ( A ) Conserved domains and putative catalytic sites of MnuA2, M. pulmonis MnuA ( WP_010925495.1 ) and mature ( N -terminal signal peptide-removed) human DNASE1 ( NP_005214.2 ). Amino acids comprising catalytic sites are indicated by red arrowheads, and two putative catalytic histidines are highlighted in red. ( B ) Purification of H262Q + H428Q mutant MnuA2 (mut-MnuA2). ( C, D ) Total RNA digestion by wild type (WT) or mutant (mut-) MnuA2 (3 nM) in the U2OS lysate. ( C ) SYBR Gold staining and ( D ) northern blotting for tRNA Gly-GCC . ( E ) in vitro DNA digestion by MnuA2. λ DNA and pcDNA3.1(+) were digested by 5 ng (roughly 1.05 nM) MnuA2.

    Article Snippet: Five nanograms of the MnuA2 were incubated at 37°C for the indicated time periods in 10 mM Tris-HCl (pH 8.0), 5 mM CaCl 2 , containing 1 μg λ phage DNA (TOYOBO), 1 μg pcDNA3.1(+) (Thermo Fisher Scientific), or 5 μg U2OS total RNA as a substrate.

    Techniques: Mutagenesis, Activity Assay, Purification, Staining, Northern Blot, In Vitro